Regina can hold up to 10 consumable items such as healing supplies and ammunition, with a separate inventory menu for key items. As such the player will always have space for any such necessary keys and the like, and inventory management is governed purely around the player's supplies. Besides Regina's inventory, there are a number of Emergency Boxes that include a significant amount of supplies for the player to collect, while also offering a place for the player to leave unwanted supplies. However unlike the item boxes in Resident Evil, the boxes are color-coded and items can only persist across multiple boxes of the same color. Furthermore, they must first be unlocked with at least one item called a 'plug', otherwise it will be inaccessible.

Mixing is the act of combining multiple supply types to increase their effectiveness. This typically revolves around mixing two small health packs to gain two medium ones for example, but it can also be used to enhance the strength of tranquilisers. Beyond mixing supplies together, there are also items that exist specifically to be mixed to increase an item's effectiveness.